› 4s10

X-ray diffraction
2.61Å resolution

Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis

Released:
DOI: 10.2210/pdb4s10/pdb
PDB entry 4s10 coloured by chain, front view.
PDB entry 4s10 coloured by chain, side view.
PDB entry 4s10 coloured by chain, top view.
Source organisms:
Primary publication:
An ER-directed gelsolin nanobody targets the first step in amyloid formation in a gelsolin amyloidosis mouse model.
Van Overbeke W, Wongsantichon J, Everaert I, Verhelle A, Zwaenepoel O, Loonchanta A, Burtnick LD, De Ganck A, Hochepied T, Haigh J, Cuvelier C, Derave W, Robinson RC, Gettemans J
Hum Mol Genet 24 2492-507 (2015)
PMID: 25601851

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209282 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GELSOLIN NANOBODY Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.7 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Gelsolin Chains: C, D
Molecule details ›
Chains: C, D
Length: 103 amino acids
Theoretical weight: 11.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 186-288; Coverage: 14%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 37.598Å b: 46.334Å c: 76.438Å
α: 103° β: 92.03° γ: 89.85°
R-values:
R R work R free
0.228 0.223 0.312
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Brain Delivery of Single-Domain Antibodies: A Focus on VHH and VNAR.
Pothin et al. (2020)
3 more

1 mention without citation

Optogenetic control of protein binding using light-switchable nanobodies.
Gil et al. (2020)