› 4tl0

X-ray diffraction
2.7Å resolution

Crystal structure of death-associated protein kinase 1 with a crucial phosphomimicking mutation

Released:
DOI: 10.2210/pdb4tl0/pdb
PDB entry 4tl0 coloured by chain, front view.
PDB entry 4tl0 coloured by chain, side view.
PDB entry 4tl0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Temmerman K, Simon B, Wilmanns M

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P53355 (Residues: 1-334; Coverage: 23%)
Gene names: DAPK, DAPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand NH4 3 x NH4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 50.34Å b: 77.794Å c: 110.123Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.218
Expression system: Escherichia coli BL21

Quick links