› 4tnd

X-ray diffraction
1.8Å resolution

Crystal Structure of G Protein-Coupled Receptor Kinase 5 in Complex with AMP-PNP

Released:
DOI: 10.2210/pdb4tnd/pdb
PDB entry 4tnd coloured by chain, front view.
PDB entry 4tnd coloured by chain, side view.
PDB entry 4tnd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Atomic Structure of GRK5 Reveals Distinct Structural Features Novel for G Protein-coupled Receptor Kinases.
Komolov KE, Bhardwaj A, Benovic JL
J Biol Chem 290 20629-20647 (2015)
PMID: 26032409

Function and Biology Details

Reaction catalysed: 
ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-coupled receptor kinase 5 Chain: A
Molecule details ›
Chain: A
Length: 590 amino acids
Theoretical weight: 67.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34947 (Residues: 1-590; Coverage: 100%)
Gene names: GPRK5, GRK5
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P43212
Unit cell:
a: 62.975Å b: 62.975Å c: 292.644Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.205
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

G protein-coupled receptor kinase 2 (GRK2) as a multifunctional signaling hub.
Penela et al. (2019)
4 more

6 mentions without citation

Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5.
Komolov et al. (2017)
5 more