› 4to4

X-ray diffraction
2.1Å resolution

Structure basis of cellular dNTP regulation, SAMHD1-GTP-dGTP-dCTP complex

Released:
DOI: 10.2210/pdb4to4/pdb
PDB entry 4to4 coloured by chain, front view.
PDB entry 4to4 coloured by chain, side view.
PDB entry 4to4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of cellular dNTP regulation by SAMHD1.
Ji X, Tang C, Zhao Q, Wang W, Xiong Y
Proc Natl Acad Sci U S A 111 E4305-14 (2014)
PMID: 25267621

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 514 amino acids
Theoretical weight: 59.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 113-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

4 bound ligands:
Ligand DCP 4 x DCP
Ligand MG 8 x MG
Ligand GTP 4 x GTP
Ligand DGT 4 x DGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 86.712Å b: 145.843Å c: 98.654Å
α: 90° β: 114.75° γ: 90°
R-values:
R R work R free
0.198 0.197 0.225
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Deoxyribonucleotide metabolism, mutagenesis and cancer.
Mathews CK. (2015)
18 more

1 mention without citation

Structural basis of cellular dNTP regulation by SAMHD1.
Ji et al. (2014)