› 4tru

X-ray diffraction
1.81Å resolution

Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor

Released:
DOI: 10.2210/pdb4tru/pdb
PDB entry 4tru coloured by chain, front view.
PDB entry 4tru coloured by chain, side view.
PDB entry 4tru coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An Analysis of MIF Structural Features that Control Functional Activation of CD74.
Pantouris G, Syed MA, Fan C, Rajasekaran D, Cho TY, Rosenberg EM, Bucala R, Bhandari V, Lolis EJ
Chem Biol 22 1197-205 (2015)
PMID: 26364929

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand IPA 3 x IPA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 67.513Å b: 67.578Å c: 88.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.19
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The biological function and significance of CD74 in immune diseases.
Su et al. (2017)
10 more

1 mention without citation

An Analysis of MIF Structural Features that Control Functional Activation of CD74.
Pantouris et al. (2015)