› 4tsf

X-ray diffraction
3.2Å resolution

The Pathway of Binding of the Intrinsically Disordered Mitochondrial Inhibitor Protein to F1-ATPase

Released:
DOI: 10.2210/pdb4tsf/pdb
PDB entry 4tsf coloured by chain, front view.
PDB entry 4tsf coloured by chain, side view.
PDB entry 4tsf coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Pathway of binding of the intrinsically disordered mitochondrial inhibitor protein to F1-ATPase.
Bason JV, Montgomery MG, Leslie AG, Walker JE
Proc Natl Acad Sci U S A 111 11305-10 (2014)
PMID: 25049402

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-133617 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 55.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P19483 (Residues: 44-553; Coverage: 92%)
Gene names: ATP5A1, ATP5A2, ATP5F1A
Sequence domains:
Structure domains:
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 480 amino acids
Theoretical weight: 51.62 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 49-528; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chain: G
Molecule details ›
Chain: G
Length: 273 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-298; Coverage: 92%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase
Structure domains:
ATPase inhibitor, mitochondrial Chains: H, I
Molecule details ›
Chains: H, I
Length: 66 amino acids
Theoretical weight: 7.46 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01096 (Residues: 26-85; Coverage: 55%)
Gene names: ATP5IF1, ATPI, ATPIF1
Sequence domains: Mitochondrial ATPase inhibitor, IATP
Structure domains: Single helix bin

Ligands and Environments

3 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 5 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 108.015Å b: 154.42Å c: 269.465Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The Mitochondrial Permeability Transition Pore: Channel Formation by F-ATP Synthase, Integration in Signal Transduction, and Role in Pathophysiology.
Bernardi et al. (2015)
13 more

3 mentions without citation

Pathway of binding of the intrinsically disordered mitochondrial inhibitor protein to F1-ATPase.
Bason et al. (2014)
2 more