› 4tt4

X-ray diffraction
2.7Å resolution

Crystal structure of ATAD2A bromodomain complexed with H3(1-21)K14Ac peptide

Released:
DOI: 10.2210/pdb4tt4/pdb
PDB entry 4tt4 coloured by chain, front view.
PDB entry 4tt4 coloured by chain, side view.
PDB entry 4tt4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Observed bromodomain flexibility reveals histone peptide- and small molecule ligand-compatible forms of ATAD2.
Poncet-Montange G, Zhan Y, Bardenhagen JP, Petrocchi A, Leo E, Shi X, Lee GR, Leonard PG, Geck Do MK, Cardozo MG, Andersen JN, Palmer WS, Jones P, Ladbury JE
Biochem J 466 337-46 (2015)
PMID: 25486442

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165195 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase family AAA domain-containing protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 15.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6PL18 (Residues: 981-1108; Coverage: 9%)
Gene names: ATAD2, L16, PRO2000
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H3(1-21)K4Ac Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 491 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 2 x CL
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 108.16Å b: 115.68Å c: 77.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Lysine Acetylation Goes Global: From Epigenetics to Metabolism and Therapeutics.
Ali et al. (2018)
6 more

4 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
3 more