› 4ttc

X-ray diffraction
2.65Å resolution

Crystal structure of homo sapiens IODOTYROSINE DEIODINASE bound to FMN and mono-iodotyrosine (MIT)

Released:
DOI: 10.2210/pdb4ttc/pdb
PDB entry 4ttc coloured by chain, front view.
PDB entry 4ttc coloured by chain, side view.
PDB entry 4ttc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A switch between one- and two-electron chemistry of the human flavoprotein iodotyrosine deiodinase is controlled by substrate.
Hu J, Chuenchor W, Rokita SE
J Biol Chem 290 590-600 (2015)
PMID: 25395621

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + NADP(+) + iodide = 3-iodo-L-tyrosine + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iodotyrosine deiodinase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 265 amino acids
Theoretical weight: 30.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PHW0 (Residues: 32-289; Coverage: 89%)
Gene names: C6orf71, DEHAL1, IYD
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments


Cofactor: Ligand FMN 6 x FMN
1 bound ligand:
Ligand IYR 6 x IYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P61
Unit cell:
a: 104.747Å b: 104.747Å c: 303.338Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.212
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
7 more

6 mentions without citation

On the Natural History of Flavin-Based Electron Bifurcation.
Baymann et al. (2018)
5 more