› 4tu8

X-ray diffraction
1.92Å resolution

STRUCTURE OF U2AF65 VARIANT WITH BRU5A6 DNA

Released:
DOI: 10.2210/pdb4tu8/pdb
PDB entry 4tu8 coloured by chain, front view.
PDB entry 4tu8 coloured by chain, side view.
PDB entry 4tu8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.
Agrawal AA, McLaughlin KJ, Jenkins JL, Kielkopf CL
Proc Natl Acad Sci U S A 111 17420-5 (2014)
PMID: 25422459

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118165 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 148-336; Coverage: 36%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*UP*UP*UP*UP*(BRU)P*DA*U)-3') Chains: E, P
Molecule details ›
Chains: E, P
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand DIO 7 x DIO
Ligand GOL 1 x GOL
Ligand EDO 9 x EDO
Ligand SO4 3 x SO4
Ligand CPQ 1 x CPQ
1 modified residue:
Ligand BRU 2 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 164.04Å b: 37.178Å c: 100.71Å
α: 90° β: 125.66° γ: 90°
R-values:
R R work R free
0.182 0.178 0.218
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Therapeutic approaches to treat human spliceosomal diseases.
DeNicola et al. (2019)
1 more

2 mentions without citation

Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.
Agrawal et al. (2014)
1 more