› 4tz7

X-ray diffraction
3.31Å resolution

Crystal structure of type I phosphatidylinositol 4-phosphate 5-kinase alpha from Zebrafish

Released:
DOI: 10.2210/pdb4tz7/pdb
PDB entry 4tz7 coloured by chain, front view.
PDB entry 4tz7 coloured by chain, side view.
PDB entry 4tz7 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Resolution of structure of PIP5K1A reveals molecular mechanism for its regulation by dimerization and dishevelled.
Hu J, Yuan Q, Kang X, Qin Y, Li L, Ha Y, Wu D
Nat Commun 6 8205 (2015)
PMID: 26365782

Function and Biology Details

Reaction catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PIPK domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.87 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q503I3 (Residues: 49-431; Coverage: 69%)
Gene names: pip5k1a, pip5k1aa, zgc:110576
Sequence domains: Phosphatidylinositol-4-phosphate 5-Kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 88.946Å b: 88.946Å c: 157.074Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.207 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Understanding phosphoinositides: rare, dynamic, and essential membrane phospholipids.
Dickson et al. (2019)
4 more

9 mentions without citation

Beyond PI3Ks: targeting phosphoinositide kinases in disease.
Burke et al. (2023)
8 more