› 4tzi

X-ray diffraction
2.1Å resolution

Structure of unliganded Lyn SH2 domain

Released:
DOI: 10.2210/pdb4tzi/pdb
PDB entry 4tzi coloured by chain, front view.
PDB entry 4tzi coloured by chain, side view.
PDB entry 4tzi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Tyrosine phosphorylation of the Lyn Src homology 2 (SH2) domain modulates its binding affinity and specificity.
Jin LL, Wybenga-Groot LE, Tong J, Taylor P, Minden MD, Trudel S, McGlade CJ, Moran MF
Mol Cell Proteomics 14 695-706 (2015)
PMID: 25587033

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase Lyn Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P25911 (Residues: 115-229; Coverage: 23%)
Gene name: Lyn
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 111.915Å b: 27.995Å c: 74.915Å
α: 90° β: 96.81° γ: 90°
R-values:
R R work R free
0.203 0.199 0.254
Expression system: Escherichia coli

Quick links

Citations

8 review citations

TCR Signaling: Mechanisms of Initiation and Propagation.
Courtney et al. (2018)
7 more

3 mentions without citation

Structural insights into redox-active cysteine residues of the Src family kinases.
Heppner DE. (2021)
2 more