› 4u18

X-ray diffraction
2.64Å resolution

Crystal structure of human peroxisomal delta3,delta2, enoyl-CoA isomerase (ISO-ECI2)

Released:
DOI: 10.2210/pdb4u18/pdb
PDB entry 4u18 coloured by chain, front view.
PDB entry 4u18 coloured by chain, side view.
PDB entry 4u18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human Δ³,Δ²-enoyl-CoA isomerase, type 2: a structural enzymology study on the catalytic role of its ACBP domain and helix-10.
Onwukwe GU, Kursula P, Koski MK, Schmitz W, Wierenga RK
FEBS J 282 746-68 (2015)
PMID: 25515061

Function and Biology Details

Reaction catalysed: 
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA delta isomerase 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 276 amino acids
Theoretical weight: 30.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75521 (Residues: 138-390; Coverage: 64%)
Gene names: DRS1, ECI2, HCA88, PECI
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 76.428Å b: 91.915Å c: 130.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Biochemistry and Physiology of Mitochondrial Fatty Acid β-Oxidation and Its Genetic Disorders.
Houten et al. (2016)
2 more

1 mention without citation

ACBD6 protein controls acyl chain availability and specificity of the N-myristoylation modification of proteins.
Soupene et al. (2019)