› 4u2u

X-ray diffraction
2.9Å resolution

Bak domain swapped dimer induced by BidBH3 with CHAPS

Released:
DOI: 10.2210/pdb4u2u/pdb
PDB entry 4u2u coloured by chain, front view.
PDB entry 4u2u coloured by chain, side view.
PDB entry 4u2u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Bak core and latch domains separate during activation, and freed core domains form symmetric homodimers.
Brouwer JM, Westphal D, Dewson G, Robin AY, Uren RT, Bartolo R, Thompson GV, Colman PM, Kluck RM, Czabotar PE
Mol Cell 55 938-946 (2014)
PMID: 25175025

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bcl-2 homologous antagonist/killer Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16611 (Residues: 23-186; Coverage: 78%)
Gene names: BAK, BAK1, BCL2L7, CDN1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 110.646Å b: 39.482Å c: 88.147Å
α: 90° β: 117.71° γ: 90°
R-values:
R R work R free
0.214 0.209 0.263
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.
Galluzzi et al. (2018)
32 more

3 mentions without citation

The deadly landscape of pro-apoptotic BCL-2 proteins in the outer mitochondrial membrane.
Luna-Vargas et al. (2016)
2 more