› 4une

X-ray diffraction
1.59Å resolution

Human insulin B26Phe mutant crystal structure

Released:
DOI: 10.2210/pdb4une/pdb
PDB entry 4une coloured by chain, front view.
PDB entry 4une coloured by chain, side view.
PDB entry 4une coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human insulin analogues modified at the B26 site reveal a hormone conformation that is undetected in the receptor complex.
Záková L, Kletvíková E, Lepšík M, Collinsová M, Watson CJ, Turkenburg JP, Jiráček J, Brzozowski AM
Acta Crystallogr D Biol Crystallogr 70 2765-74 (2014)
PMID: 25286859

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23
Spacegroup: P212121
Unit cell:
a: 43.887Å b: 45.761Å c: 51.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.18
Expression system: Not provided

Quick links

Citations

4 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
3 more

1 mention without citation

Human insulin analogues modified at the B26 site reveal a hormone conformation that is undetected in the receptor complex.
Záková et al. (2014)