› 4unk

X-ray diffraction
2Å resolution

Crystal structure of human triosephosphate isomerase (mutant N15D)

Released:
DOI: 10.2210/pdb4unk/pdb
PDB entry 4unk coloured by chain, front view.
PDB entry 4unk coloured by chain, side view.
PDB entry 4unk coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: DeLaMora-DeLaMora I, Torres-Larios A, Enriquez-Flores S, Mendez ST, Castillo-Villanueva A, Gomez-Manzo S, Lopez-Velazquez G, Marcial-Quino J, Torres-Arroyo A, Garcia-Torres I, Reyes-Vivas H, Oria-Hernandez J

Function and Biology Details

Reactions catalysed: 
Glycerone phosphate = methylglyoxal + phosphate
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60174 (Residues: 2-249; Coverage: 100%)
Gene names: TPI, TPI1
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 47.628Å b: 70.714Å c: 146.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links