› 4unt

X-ray diffraction
2.7Å resolution

Induced monomer of the Mcg variable domain

Released:
DOI: 10.2210/pdb4unt/pdb
PDB entry 4unt coloured by chain, front view.
PDB entry 4unt coloured by chain, side view.
PDB entry 4unt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Formation of amyloid fibers by monomeric light chain variable domains.
Brumshtein B, Esswein SR, Landau M, Ryan CM, Whitelegge JP, Phillips ML, Cascio D, Sawaya MR, Eisenberg DS
J Biol Chem 289 27513-25 (2014)
PMID: 25138218

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin lambda variable 2-8 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 111 amino acids
Theoretical weight: 11.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01709 (Residues: 20-118; Coverage: 100%)
Gene name: IGLV2-8
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 103.13Å b: 90.35Å c: 99.17Å
α: 90° β: 118.86° γ: 90°
R-values:
R R work R free
0.246 0.243 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Screening methods for identifying pharmacological chaperones.
Shin et al. (2017)
1 more

2 mentions without citation

Formation of amyloid fibers by monomeric light chain variable domains.
Brumshtein et al. (2014)
1 more