› 4uqi

X-ray diffraction
2.79Å resolution

AP2 controls clathrin polymerization with a membrane-activated switch

Released:
DOI: 10.2210/pdb4uqi/pdb
PDB entry 4uqi coloured by chain, front view.
PDB entry 4uqi coloured by chain, side view.
PDB entry 4uqi coloured by chain, top view.
Source organisms:
Primary publication:
Clathrin adaptors. AP2 controls clathrin polymerization with a membrane-activated switch.
Kelly BT, Graham SC, Liska N, Dannhauser PN, Höning S, Ungewickell EJ, Owen DJ
Science 345 459-63 (2014)
PMID: 25061211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148381 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 70.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18484 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 657 amino acids
Theoretical weight: 73.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63010 (Residues: 1-651; Coverage: 70%)
Gene names: ADTB2, AP2B1, CLAPB1
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 446 amino acids
Theoretical weight: 51.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 1-236, 237-435; Coverage: 100%)
Gene name: Ap2m1
Sequence domains:
Structure domains:
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3121
Unit cell:
a: 121.3Å b: 121.3Å c: 259.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Mechanisms of clathrin-mediated endocytosis.
Kaksonen et al. (2018)
40 more

5 mentions without citation

A Recurrent Missense Variant in AP2M1 Impairs Clathrin-Mediated Endocytosis and Causes Developmental and Epileptic Encephalopathy.
Helbig et al. (2019)
4 more