› 4ut3

X-ray diffraction
2.19Å resolution

X-ray structure of the human PP1 gamma catalytic subunit treated with hydrogen peroxide

Released:
DOI: 10.2210/pdb4ut3/pdb
PDB entry 4ut3 coloured by chain, front view.
PDB entry 4ut3 coloured by chain, side view.
PDB entry 4ut3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeted redox inhibition of protein phosphatase 1 by Nox4 regulates eIF2α-mediated stress signaling.
Santos CX, Hafstad AD, Beretta M, Zhang M, Molenaar C, Kopec J, Fotinou D, Murray TV, Cobb AM, Martin D, Zeh Silva M, Anilkumar N, Schröder K, Shanahan CM, Brewer AC, Brandes RP, Blanc E, Parsons M, Belousov V, Cammack R, Hider RC, Steiner RA, Shah AM
EMBO J 35 319-34 (2016)
PMID: 26742780

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 37.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P36873 (Residues: 1-323; Coverage: 100%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 323 amino acids
Theoretical weight: 37.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P36873 (Residues: 1-323; Coverage: 100%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand PO4 2 x PO4
1 modified residue:
Ligand CSO 3 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 38.45Å b: 105.22Å c: 89.82Å
α: 90° β: 90.34° γ: 90°
R-values:
R R work R free
0.175 0.173 0.211
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

26 review citations

Reactive oxygen species (ROS) as pleiotropic physiological signalling agents.
Sies et al. (2020)
25 more

3 mentions without citation

Targeted redox inhibition of protein phosphatase 1 by Nox4 regulates eIF2α-mediated stress signaling.
Santos et al. (2016)
2 more