› 4uu6

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THE PDZ DOMAIN OF PALS1

Released:
DOI: 10.2210/pdb4uu6/pdb
PDB entry 4uu6 coloured by chain, front view.
PDB entry 4uu6 coloured by chain, side view.
PDB entry 4uu6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the human Pals1 PDZ domain with and without ligand suggest gated access of Crb to the PDZ peptide-binding groove.
Ivanova ME, Fletcher GC, O'Reilly N, Purkiss AG, Thompson BJ, McDonald NQ
Acta Crystallogr D Biol Crystallogr 71 555-64 (2015)
PMID: 25760605

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PALS1 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N3R9 (Residues: 251-335; Coverage: 13%)
Gene names: MPP5, PALS1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P43212
Unit cell:
a: 44.14Å b: 44.14Å c: 89.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Synthetic Protein Scaffolds Based on Peptide Motifs and Cognate Adaptor Domains for Improving Metabolic Productivity.
Horn et al. (2015)
1 more

5 mentions without citation

Structural Features of Tight-Junction Proteins.
Heinemann et al. (2019)
4 more