› 4uuk

Electron Microscopy
12.5Å resolution

Human dynamin 1 K44A superconstricted polymer stabilized with GTP strand 2

Released:
DOI: 10.2210/pdb4uuk/pdb
PDB entry 4uuk coloured by chain, front view.
PDB entry 4uuk coloured by chain, side view.
PDB entry 4uuk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A dynamin mutant defines a superconstricted prefission state.
Sundborger AC, Fang S, Heymann JA, Ray P, Chappie JS, Hinshaw JE
Cell Rep 8 734-42 (2014)
PMID: 25088425
Related structures: EMD-2701

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170161 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynamin-1 Chains: A, D, G, K
Molecule details ›
Chains: A, D, G, K
Length: 864 amino acids
Theoretical weight: 97.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05193 (Residues: 1-864; Coverage: 100%)
Gene names: DNM, DNM1
Sequence domains:
Dynamin-1 Chains: B, C, E, F, H, I, J, L
Molecule details ›
Chains: B, C, E, F, H, I, J, L
Length: 864 amino acids
Theoretical weight: 97.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05193 (Residues: 1-864; Coverage: 100%)
Gene names: DNM, DNM1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12.5Å
Relevant EMDB volumes: EMD-2701
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

11 review citations

Membrane fission by dynamin: what we know and what we need to know.
Antonny et al. (2016)
10 more