› 4uyz

X-ray diffraction
2.8Å resolution

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM II - 2.8A

Released:
DOI: 10.2210/pdb4uyz/pdb
PDB entry 4uyz coloured by chain, front view.
PDB entry 4uyz coloured by chain, side view.
PDB entry 4uyz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Notum deacylates Wnt proteins to suppress signalling activity.
Kakugawa S, Langton PF, Zebisch M, Howell S, Chang TH, Liu Y, Feizi T, Bineva G, O'Reilly N, Snijders AP, Jones EY, Vincent JP
Nature 519 187-192 (2015)
PMID: 25731175

Function and Biology Details

Reaction catalysed: 
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H(2)O = [Wnt]-L-serine + (9Z)-hexadec-9-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180133 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Palmitoleoyl-protein carboxylesterase NOTUM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 471 amino acids
Theoretical weight: 53.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P988 (Residues: 38-496; Coverage: 96%)
Gene names: NOTUM, OK/SW-CL.30
Sequence domains: Pectinacetylesterase
POLY ALA Chain: E
Molecule details ›
Chain: E
Length: 10 amino acids
Theoretical weight: 729 Da
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 60.829Å b: 193.88Å c: 75.717Å
α: 90° β: 91.88° γ: 90°
R-values:
R R work R free
0.249 0.248 0.293
Expression system: Homo sapiens

Quick links

Citations

70 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
69 more

3 mentions without citation

Notum deacylates Wnt proteins to suppress signalling activity.
Kakugawa et al. (2015)
2 more