› 4v2o

X-ray diffraction
2.13Å resolution

Structure of saposin B in complex with chloroquine

Released:
DOI: 10.2210/pdb4v2o/pdb
PDB entry 4v2o coloured by chain, front view.
PDB entry 4v2o coloured by chain, side view.
PDB entry 4v2o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Lysosomal Protein Saposin B Binds Chloroquine.
Huta BP, Mehlenbacher MR, Nie Y, Lai X, Zubieta C, Bou-Abdallah F, Doyle RP
ChemMedChem 11 277-82 (2016)
PMID: 26616259

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Saposin-B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 82 amino acids
Theoretical weight: 9.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07602 (Residues: 195-273; Coverage: 16%)
Gene names: GLBA, PSAP, SAP1
Sequence domains: Saposin-like type B, region 2
Structure domains: Saposin-like

Ligands and Environments

1 bound ligand:
Ligand CLQ 2 x CLQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 75.299Å b: 75.299Å c: 94.716Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.229 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

New Insights on the Uptake and Trafficking of Coenzyme Q.
Guile et al. (2023)
8 other articles

10 mentions without citation

Structural and molecular modelling studies reveal a new mechanism of action of chloroquine and hydroxychloroquine against SARS-CoV-2 infection.
Fantini et al. (2020)
9 more