› 4v92

Electron Microscopy
3.7Å resolution

Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES

Released:
DOI: 10.2210/pdb4v92/pdb
PDB entry 4v92 coloured by chain, front view.
PDB entry 4v92 coloured by chain, side view.
PDB entry 4v92 coloured by chain, top view.
Source organism: Kluyveromyces lactis
Primary publication:
Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome.
Fernández IS, Bai XC, Murshudov G, Scheres SH, Ramakrishnan V
Cell 157 823-31 (2014)
PMID: 24792965
Related structures: EMD-2604

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-165271 (preferred)
Entry contents:
33 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (35 distinct):
US2 Chain: BA
Molecule details ›
Chain: BA
Length: 206 amino acids
Theoretical weight: 22.81 KDa
Source organism: Kluyveromyces lactis
ES1 Chain: BB
Molecule details ›
Chain: BB
Length: 213 amino acids
Theoretical weight: 23.98 KDa
Source organism: Kluyveromyces lactis
US5 Chain: BC
Molecule details ›
Chain: BC
Length: 216 amino acids
Theoretical weight: 23.08 KDa
Source organism: Kluyveromyces lactis
US3 Chain: BD
Molecule details ›
Chain: BD
Length: 222 amino acids
Theoretical weight: 24.63 KDa
Source organism: Kluyveromyces lactis
ES4 Chain: BE
Molecule details ›
Chain: BE
Length: 260 amino acids
Theoretical weight: 29.34 KDa
Source organism: Kluyveromyces lactis
US7 Chain: BF
Molecule details ›
Chain: BF
Length: 206 amino acids
Theoretical weight: 22.82 KDa
Source organism: Kluyveromyces lactis
ES6 Chain: BG
Molecule details ›
Chain: BG
Length: 226 amino acids
Theoretical weight: 25.78 KDa
Source organism: Kluyveromyces lactis
ES7 Chain: BH
Molecule details ›
Chain: BH
Length: 184 amino acids
Theoretical weight: 21 KDa
Source organism: Kluyveromyces lactis
ES8 Chain: BI
Molecule details ›
Chain: BI
Length: 187 amino acids
Theoretical weight: 21.02 KDa
Source organism: Kluyveromyces lactis
US4 Chain: BJ
Molecule details ›
Chain: BJ
Length: 179 amino acids
Theoretical weight: 20.61 KDa
Source organism: Kluyveromyces lactis
ES10 Chain: BK
Molecule details ›
Chain: BK
Length: 93 amino acids
Theoretical weight: 11.05 KDa
Source organism: Kluyveromyces lactis
US17 Chain: BL
Molecule details ›
Chain: BL
Length: 142 amino acids
Theoretical weight: 16.27 KDa
Source organism: Kluyveromyces lactis
ES12 Chain: BM
Molecule details ›
Chain: BM
Length: 120 amino acids
Theoretical weight: 12.93 KDa
Source organism: Kluyveromyces lactis
US15 Chain: BN
Molecule details ›
Chain: BN
Length: 150 amino acids
Theoretical weight: 16.93 KDa
Source organism: Kluyveromyces lactis
US11 Chain: BO
Molecule details ›
Chain: BO
Length: 127 amino acids
Theoretical weight: 13.45 KDa
Source organism: Kluyveromyces lactis
US19 Chain: BP
Molecule details ›
Chain: BP
Length: 115 amino acids
Theoretical weight: 13.06 KDa
Source organism: Kluyveromyces lactis
US9 Chain: BQ
Molecule details ›
Chain: BQ
Length: 140 amino acids
Theoretical weight: 15.33 KDa
Source organism: Kluyveromyces lactis
ES17 Chain: BR
Molecule details ›
Chain: BR
Length: 121 amino acids
Theoretical weight: 13.88 KDa
Source organism: Kluyveromyces lactis
US13 Chain: BS
Molecule details ›
Chain: BS
Length: 140 amino acids
Theoretical weight: 16.34 KDa
Source organism: Kluyveromyces lactis
ES19 Chain: BT
Molecule details ›
Chain: BT
Length: 142 amino acids
Theoretical weight: 15.71 KDa
Source organism: Kluyveromyces lactis
US10 Chain: BU
Molecule details ›
Chain: BU
Length: 104 amino acids
Theoretical weight: 11.82 KDa
Source organism: Kluyveromyces lactis
ES21 Chain: BV
Molecule details ›
Chain: BV
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Kluyveromyces lactis
US8 Chain: BW
Molecule details ›
Chain: BW
Length: 129 amino acids
Theoretical weight: 14.52 KDa
Source organism: Kluyveromyces lactis
US12 Chain: BX
Molecule details ›
Chain: BX
Length: 142 amino acids
Theoretical weight: 15.65 KDa
Source organism: Kluyveromyces lactis
ES24 Chain: BY
Molecule details ›
Chain: BY
Length: 134 amino acids
Theoretical weight: 15.23 KDa
Source organism: Kluyveromyces lactis
ES25 Chain: BZ
Molecule details ›
Chain: BZ
Length: 64 amino acids
Theoretical weight: 7.37 KDa
Source organism: Kluyveromyces lactis
ES26 Chain: Ba
Molecule details ›
Chain: Ba
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Kluyveromyces lactis
ES27 Chain: Bb
Molecule details ›
Chain: Bb
Length: 81 amino acids
Theoretical weight: 8.76 KDa
Source organism: Kluyveromyces lactis
ES28 Chain: Bc
Molecule details ›
Chain: Bc
Length: 63 amino acids
Theoretical weight: 7.12 KDa
Source organism: Kluyveromyces lactis
US14 Chain: Bd
Molecule details ›
Chain: Bd
Length: 52 amino acids
Theoretical weight: 6.21 KDa
Source organism: Kluyveromyces lactis
ES30 Chain: Be
Molecule details ›
Chain: Be
Length: 55 amino acids
Theoretical weight: 6.28 KDa
Source organism: Kluyveromyces lactis
ES31 Chain: Bf
Molecule details ›
Chain: Bf
Length: 64 amino acids
Theoretical weight: 6.57 KDa
Source organism: Kluyveromyces lactis
RACK1 Chain: Bg
Molecule details ›
Chain: Bg
Length: 315 amino acids
Theoretical weight: 34.44 KDa
Source organism: Kluyveromyces lactis
18S Ribosomal RNA Chain: A2
Molecule details ›
Chain: A2
Length: 1767 nucleotides
Theoretical weight: 569.19 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
RNA OF CRICKET-PARALYSIS-VIRUS-IRES Chain: AZ
Molecule details ›
Chain: AZ
Length: 190 nucleotides
Theoretical weight: 60.77 KDa
Sequence domains: Cripavirus internal ribosome entry site (IRES)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-2604

Quick links

Citations

39 review citations

How cryo-EM is revolutionizing structural biology.
Bai et al. (2015)
38 more

7 mentions without citation

Dynamics of IRES-mediated translation.
Johnson et al. (2017)
6 more