› 4w5n

X-ray diffraction
2.9Å resolution

The Crystal Structure of Human Argonaute2 Bound to a Defined Guide RNA

Released:
DOI: 10.2210/pdb4w5n/pdb
PDB entry 4w5n coloured by chain, front view.
PDB entry 4w5n coloured by chain, side view.
PDB entry 4w5n coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for microRNA targeting.
Schirle NT, Sheu-Gruttadauria J, MacRae IJ
Science 346 608-13 (2014)
PMID: 25359968

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193868 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 97.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
RNA (5'-R(P*UP*UP*CP*AP*CP*AP*UP*UP*CP*AP*AP*GP*UP*CP*UP*CP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.55 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IPH 3 x IPH
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 63.117Å b: 108.87Å c: 68.071Å
α: 90° β: 106.93° γ: 90°
R-values:
R R work R free
0.219 0.217 0.253
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

80 review citations

Metazoan MicroRNAs.
Bartel DP. (2018)
79 more

23 mentions without citation

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?
Nakanishi K. (2016)
22 more