› 4wb6

X-ray diffraction
2.1Å resolution

Crystal structure of a L205R mutant of human cAMP-dependent protein kinase A (catalytic alpha subunit)

Released:
DOI: 10.2210/pdb4wb6/pdb
PDB entry 4wb6 coloured by chain, front view.
PDB entry 4wb6 coloured by chain, side view.
PDB entry 4wb6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into mis-regulation of protein kinase A in human tumors.
Cheung J, Ginter C, Cassidy M, Franklin MC, Rudolph MJ, Robine N, Darnell RB, Hendrickson WA
Proc Natl Acad Sci U S A 112 1374-9 (2015)
PMID: 25605907

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148114 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17612 (Residues: 2-351; Coverage: 100%)
Gene names: PKACA, PRKACA
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase catalytic subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 40.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17612 (Residues: 2-351; Coverage: 100%)
Gene names: PKACA, PRKACA
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chains: I, J
Molecule details ›
Chains: I, J
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61925 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 4 x MG
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 56.169Å b: 90.193Å c: 90.377Å
α: 90° β: 96.12° γ: 90°
R-values:
R R work R free
0.19 0.188 0.237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology.
Turnham et al. (2016)
13 more

6 mentions without citation

Structural insights into mis-regulation of protein kinase A in human tumors.
Cheung et al. (2015)
5 more