› 4wbh

X-ray diffraction
2.2Å resolution

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM I APO - 2.2A

Released:
DOI: 10.2210/pdb4wbh/pdb
PDB entry 4wbh coloured by chain, front view.
PDB entry 4wbh coloured by chain, side view.
PDB entry 4wbh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Notum deacylates Wnt proteins to suppress signalling activity.
Kakugawa S, Langton PF, Zebisch M, Howell S, Chang TH, Liu Y, Feizi T, Bineva G, O'Reilly N, Snijders AP, Jones EY, Vincent JP
Nature 519 187-192 (2015)
PMID: 25731175

Function and Biology Details

Reaction catalysed: 
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H(2)O = [Wnt]-L-serine + (9Z)-hexadec-9-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Palmitoleoyl-protein carboxylesterase NOTUM Chains: A, B
Molecule details ›
Chains: A, B
Length: 471 amino acids
Theoretical weight: 53.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P988 (Residues: 38-496; Coverage: 96%)
Gene names: NOTUM, OK/SW-CL.30
Sequence domains: Pectinacetylesterase

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand NAG 1 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 72.702Å b: 175.433Å c: 60.764Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.262
Expression system: Homo sapiens

Quick links

Citations

69 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
68 more

3 mentions without citation

Ghrelin octanoylation by ghrelin O-acyltransferase: protein acylation impacting metabolic and neuroendocrine signalling.
Davis et al. (2021)
2 more