› 4wh7

X-ray diffraction
1.62Å resolution

Structure of the CDC25B Phosphatase Catalytic Domain with Bound Ligand

Released:
DOI: 10.2210/pdb4wh7/pdb
PDB entry 4wh7 coloured by chain, front view.
PDB entry 4wh7 coloured by chain, side view.
PDB entry 4wh7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition of CDC25B phosphatase through disruption of protein-protein interaction.
Lund G, Dudkin S, Borkin D, Ni W, Grembecka J, Cierpicki T
ACS Chem Biol 10 390-4 (2015)
PMID: 25423142

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30305 (Residues: 386-565; Coverage: 31%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand 8H8 1 x 8H8
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.207Å b: 71.442Å c: 73.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.198
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phosphatases and kinases regulating CDC25 activity in the cell cycle: clinical implications of CDC25 overexpression and potential treatment strategies.
Sur et al. (2016)
4 more

1 mention without citation

Inhibition of CDC25B phosphatase through disruption of protein-protein interaction.
Lund et al. (2015)