› 4wii

X-ray diffraction
2.05Å resolution

HUMAN SPLICING FACTOR, CONSTRUCT 3

Released:
DOI: 10.2210/pdb4wii/pdb
PDB entry 4wii coloured by chain, front view.
PDB entry 4wii coloured by chain, side view.
PDB entry 4wii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation.
Lee M, Sadowska A, Bekere I, Ho D, Gully BS, Lu Y, Iyer KS, Trewhella J, Fox AH, Bond CS
Nucleic Acids Res 43 3826-40 (2015)
PMID: 25765647

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor, proline- and glutamine-rich Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23246 (Residues: 276-535; Coverage: 37%)
Gene names: PSF, SFPQ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 63.447Å b: 67.611Å c: 119.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.235
Expression system: Escherichia coli

Quick links

Citations

17 review citations

RNA splicing factors as oncoproteins and tumour suppressors.
Dvinge et al. (2016)
16 more

8 mentions without citation

The DBHS proteins SFPQ, NONO and PSPC1: a multipurpose molecular scaffold.
Knott et al. (2016)
7 more