› 4wik

X-ray diffraction
3Å resolution

HUMAN SPLICING FACTOR, CONSTRUCT 2

Released:
DOI: 10.2210/pdb4wik/pdb
PDB entry 4wik coloured by chain, front view.
PDB entry 4wik coloured by chain, side view.
PDB entry 4wik coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation.
Lee M, Sadowska A, Bekere I, Ho D, Gully BS, Lu Y, Iyer KS, Trewhella J, Fox AH, Bond CS
Nucleic Acids Res 43 3826-40 (2015)
PMID: 25765647

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor, proline- and glutamine-rich Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 28.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23246 (Residues: 369-598; Coverage: 33%)
Gene names: PSF, SFPQ
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 127.399Å b: 180.416Å c: 57.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.291
Expression system: Escherichia coli

Quick links

Citations

17 review citations

RNA splicing factors as oncoproteins and tumour suppressors.
Dvinge et al. (2016)
16 more

3 mentions without citation

The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation.
Lee et al. (2015)
2 more