› 4wk8

X-ray diffraction
3.4Å resolution

FOXP3 forms a domain-swapped dimer to bridge DNA

Released:
DOI: 10.2210/pdb4wk8/pdb
PDB entry 4wk8 coloured by chain, front view.
PDB entry 4wk8 coloured by chain, side view.
PDB entry 4wk8 coloured by chain, top view.
Source organisms:
Primary publication:
DNA binding by FOXP3 domain-swapped dimer suggests mechanisms of long-range chromosomal interactions.
Chen Y, Chen C, Zhang Z, Liu CC, Johnson ME, Espinoza CA, Edsall LE, Ren B, Zhou XJ, Grant SF, Wells AD, Chen L
Nucleic Acids Res 43 1268-82 (2015)
PMID: 25567984

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118224 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein P3 41 kDa form Chains: F, G
Molecule details ›
Chains: F, G
Length: 82 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZS1 (Residues: 336-417; Coverage: 19%)
Gene names: FOXP3, IPEX, JM2
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*TP*AP*GP*GP*AP*AP*AP*AP*TP*TP*TP*GP*TP*TP*TP*CP*AP*TP*AP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*AP*CP*TP*AP*TP*GP*AP*AP*AP*CP*AP*AP*AP*TP*TP*TP*TP*CP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21212
Unit cell:
a: 81.431Å b: 84.837Å c: 68.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.19 0.252
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Molecular networks of FOXP family: dual biologic functions, interplay with other molecules and clinical implications in cancer progression.
Kim et al. (2019)
13 more

7 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
6 more