› 4wo4

X-ray diffraction
2.5Å resolution

The molecular bases of Delta/Alpha beta T cell-mediated antigen recognition.

Released:
DOI: 10.2210/pdb4wo4/pdb
PDB entry 4wo4 coloured by chain, front view.
PDB entry 4wo4 coloured by chain, side view.
PDB entry 4wo4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular bases of δ/αβ T cell-mediated antigen recognition.
Pellicci DG, Uldrich AP, Le Nours J, Ross F, Chabrol E, Eckle SB, de Boer R, Lim RT, McPherson K, Besra G, Howell AR, Moretta L, McCluskey J, Heemskerk MH, Gras S, Rossjohn J, Godfrey DI
J Exp Med 211 2599-615 (2014)
PMID: 25452463

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210660 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Antigen-presenting glycoprotein CD1d Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15813 (Residues: 24-295; Coverage: 86%)
Gene name: CD1D
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
TCR variable DELTA 1 CHAIN and TCR constant Alpha Chain: C
Molecule details ›
Chain: C
Length: 207 amino acids
Theoretical weight: 23.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
TCR variable BETA 2 (TRVB20) chain and TCR constant BETA Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand JLS 1 x JLS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.93Å b: 79.25Å c: 189.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.237
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Human gamma delta T cells: Evolution and ligand recognition.
Adams et al. (2015)
16 more

5 mentions without citation

The promise of γδ T cells and the γδ T cell receptor for cancer immunotherapy.
Legut et al. (2015)
4 more