› 4wp0

X-ray diffraction
3Å resolution

Crystal structure of human kynurenine aminotransferase-I with a C-terminal V5-hexahistidine tag

Released:
DOI: 10.2210/pdb4wp0/pdb
PDB entry 4wp0 coloured by chain, front view.
PDB entry 4wp0 coloured by chain, side view.
PDB entry 4wp0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Nadvi NA, Salam NK, Park J, Akladios FN, Kapoor V, Collyer CA, Gorrell MD, Church WB

Function and Biology Details

Reactions catalysed: 
L-glutamine + phenylpyruvate = 2-oxoglutaramate + L-phenylalanine
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine--oxoglutarate transaminase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 472 amino acids
Theoretical weight: 53.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16773 (Residues: 1-422; Coverage: 100%)
Gene names: CCBL1, KYAT1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P61
Unit cell:
a: 231.15Å b: 231.15Å c: 56.105Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.266
Expression system: Spodoptera frugiperda

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