4wqo

X-ray diffraction
3.2Å resolution

Structure of VHL-EloB-EloC-Cul2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154085 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
von Hippel-Lindau disease tumor suppressor Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40337 (Residues: 1-213; Coverage: 100%)
Gene name: VHL
Sequence domains:
Structure domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Cullin-2 Chain: D
Molecule details ›
Chain: D
Length: 186 amino acids
Theoretical weight: 22.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13617 (Residues: 1-163; Coverage: 22%)
Gene name: CUL2
Structure domains: Cullin Repeats

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 108.278Å b: 108.278Å c: 213.768Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.221 0.25
Expression system: Escherichia coli