› 4wrn

X-ray diffraction
3.2Å resolution

Crystal structure of the polymerization region of human uromodulin/Tamm-Horsfall protein

Released:
DOI: 10.2210/pdb4wrn/pdb
PDB entry 4wrn coloured by chain, front view.
PDB entry 4wrn coloured by chain, side view.
PDB entry 4wrn coloured by chain, top view.
Source organisms:
Primary publication:
A structured interdomain linker directs self-polymerization of human uromodulin.
Bokhove M, Nishimura K, Brunati M, Han L, de Sanctis D, Rampoldi L, Jovine L
Proc Natl Acad Sci U S A 113 1552-7 (2016)
PMID: 26811476

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Uromodulin, secreted form Chains: A, B
Molecule details ›
Chains: A, B
Length: 695 amino acids
Theoretical weight: 76.54 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P0AEY0 (Residues: 27-393; Coverage: 99%)
  • Canonical: P07911 (Residues: 295-610; Coverage: 51%)
Gene names: ECs5017, UMOD, Z5632, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 242.32Å b: 242.32Å c: 258.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.221 0.246
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Isolation of Extracellular Vesicles: General Methodologies and Latest Trends.
Konoshenko et al. (2018)
14 more

9 mentions without citation

A structured interdomain linker directs self-polymerization of human uromodulin.
Bokhove et al. (2016)
8 more