› 4wtw

X-ray diffraction
1.61Å resolution

Crystal structure of the third FnIII domain of integrin beta4

Released:
DOI: 10.2210/pdb4wtw/pdb
PDB entry 4wtw coloured by chain, front view.
PDB entry 4wtw coloured by chain, side view.
PDB entry 4wtw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4.
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM
Acta Crystallogr D Biol Crystallogr 71 969-85 (2015)
PMID: 25849406

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrin beta-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16144 (Residues: 1527-1618; Coverage: 5%)
Gene name: ITGB4
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand 1PE 1 x 1PE
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I212121
Unit cell:
a: 66.76Å b: 68.37Å c: 88.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The opposing roles of laminin-binding integrins in cancer.
Ramovs et al. (2017)
1 more

3 mentions without citation

MMM: A toolbox for integrative structure modeling.
Jeschke G. (2018)
2 more