› 4wzi

X-ray diffraction
2.58Å resolution

Crystal structure of crosslink stabilized long-form PDE4B

Released:
DOI: 10.2210/pdb4wzi/pdb
PDB entry 4wzi coloured by chain, front view.
PDB entry 4wzi coloured by chain, side view.
PDB entry 4wzi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineered stabilization and structural analysis of the autoinhibited conformation of PDE4.
Cedervall P, Aulabaugh A, Geoghegan KF, McLellan TJ, Pandit J
Proc Natl Acad Sci U S A 112 E1414-22 (2015)
PMID: 25775568

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-AMP phosphodiesterase 4B Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 74.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07343 (Residues: 122-736; Coverage: 84%)
Gene names: DPDE4, PDE4B
Sequence domains:
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

4 bound ligands:
Ligand ZN 3 x ZN
Ligand NA 2 x NA
Ligand MG 2 x MG
Ligand IOD 2 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P3221
Unit cell:
a: 137.83Å b: 137.83Å c: 141.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.206
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Phosphodiesterase-4 inhibition as a therapeutic strategy for metabolic disorders.
Wu et al. (2016)
4 more

3 mentions without citation

Genetic Association of Phosphodiesterases With Human Cognitive Performance.
Gurney ME. (2019)
2 more