› 4x09

X-ray diffraction
1.72Å resolution

Structure of human RNase 6 in complex with sulphate anions

Released:
DOI: 10.2210/pdb4x09/pdb
PDB entry 4x09 coloured by chain, front view.
PDB entry 4x09 coloured by chain, side view.
PDB entry 4x09 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The first crystal structure of human RNase 6 reveals a novel substrate-binding and cleavage site arrangement.
Prats-Ejarque G, Arranz-Trullén J, Blanco JA, Pulido D, Nogués MV, Moussaoui M, Boix E
Biochem J 473 1523-36 (2016)
PMID: 27013146

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease K6 Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93091 (Residues: 24-150; Coverage: 100%)
Gene names: RNASE6, RNS6
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 27.733Å b: 38.86Å c: 97.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Immune Modulation by Human Secreted RNases at the Extracellular Space.
Lu et al. (2018)
2 more

5 mentions without citation

Insights into the Antimicrobial Mechanism of Action of Human RNase6: Structural Determinants for Bacterial Cell Agglutination and Membrane Permeation.
Pulido et al. (2016)
4 more