› 4x1h

X-ray diffraction
2.29Å resolution

Opsin/G(alpha) peptide complex stabilized by nonyl-glucoside

Released:
DOI: 10.2210/pdb4x1h/pdb
PDB entry 4x1h coloured by chain, front view.
PDB entry 4x1h coloured by chain, side view.
PDB entry 4x1h coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The High-Resolution Structure of Activated Opsin Reveals a Conserved Solvent Network in the Transmembrane Region Essential for Activation.
Blankenship E, Vahedi-Faridi A, Lodowski DT
Structure 23 2358-2364 (2015)
PMID: 26526852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136073 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
C-terminal derived peptide of guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand BNG 2 x BNG
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 242.136Å b: 242.136Å c: 109.721Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.217 0.228
Expression system: Not provided

Quick links

Citations

8 review citations

What Do Structures Tell Us About Chemokine Receptor Function and Antagonism?
Kufareva et al. (2017)
7 more

11 mentions without citation

Structural Basis for G Protein-Coupled Receptor Activation.
Manglik et al. (2017)
10 more