› 4x1m

X-ray diffraction
2.17Å resolution

Structural basis for mutation-induced destabilization of Profilin 1 in ALS

Released:
DOI: 10.2210/pdb4x1m/pdb
PDB entry 4x1m coloured by chain, front view.
PDB entry 4x1m coloured by chain, side view.
PDB entry 4x1m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for mutation-induced destabilization of profilin 1 in ALS.
Boopathy S, Silvas TV, Tischbein M, Jansen S, Shandilya SM, Zitzewitz JA, Landers JE, Goode BL, Schiffer CA, Bosco DA
Proc Natl Acad Sci U S A 112 7984-9 (2015)
PMID: 26056300

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Profilin-1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07737 (Residues: 1-140; Coverage: 100%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 73.65Å b: 31.712Å c: 60.539Å
α: 90° β: 122.03° γ: 90°
R-values:
R R work R free
0.197 0.196 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The Actin Cytoskeleton in SMA and ALS: How Does It Contribute to Motoneuron Degeneration?
Hensel et al. (2018)
9 more

2 mentions without citation

Structural basis for mutation-induced destabilization of profilin 1 in ALS.
Boopathy et al. (2015)
1 more