› 4x44

X-ray diffraction
2.05Å resolution

Crystal Structure of Mutant R89Q of human Adenine phosphoribosyltransferase

Released:
DOI: 10.2210/pdb4x44/pdb
PDB entry 4x44 coloured by chain, front view.
PDB entry 4x44 coloured by chain, side view.
PDB entry 4x44 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pimenta A, Pereira HM, Mercaldi G, Thiemann OH

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 19.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07741 (Residues: 1-180; Coverage: 100%)
Gene name: APRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand AMP 1 x AMP
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P43212
Unit cell:
a: 59.493Å b: 59.493Å c: 109.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.203
Expression system: Escherichia coli BL21(DE3)

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