› 4x45

X-ray diffraction
1.75Å resolution

Crystal Structure of F173G Mutant of Human APRT

Released:
DOI: 10.2210/pdb4x45/pdb
PDB entry 4x45 coloured by chain, front view.
PDB entry 4x45 coloured by chain, side view.
PDB entry 4x45 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pereira HM, Pimenta A, Mercaldi G, Thiemann OH

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07741 (Residues: 1-180; Coverage: 100%)
Gene name: APRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P212121
Unit cell:
a: 47.748Å b: 140.617Å c: 49.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.199
Expression system: Escherichia coli BL21(DE3)

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