› 4x4m

X-ray diffraction
3.48Å resolution

Structure of FcgammaRI in complex with Fc reveals the importance of glycan recognition for high affinity IgG binding

Released:
DOI: 10.2210/pdb4x4m/pdb
PDB entry 4x4m coloured by chain, front view.
PDB entry 4x4m coloured by chain, side view.
PDB entry 4x4m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of FcγRI in complex with Fc reveals the importance of glycan recognition for high-affinity IgG binding.
Lu J, Chu J, Zou Z, Hamacher NB, Rixon MW, Sun PD
Proc Natl Acad Sci U S A 112 833-8 (2015)
PMID: 25561553

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134855 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 219 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 112-328; Coverage: 54%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
High affinity immunoglobulin gamma Fc receptor I Chains: E, F
Molecule details ›
Chains: E, F
Length: 275 amino acids
Theoretical weight: 31.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12314 (Residues: 21-289; Coverage: 75%)
Gene names: FCG1, FCGR1, FCGR1A, IGFR1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUL, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUL, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, SIA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand FUL 1 x FUL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 59.921Å b: 67.991Å c: 125.028Å
α: 89.86° β: 112.25° γ: 89.96°
R-values:
R R work R free
0.249 0.245 0.296
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

38 review citations

Molecular properties of human IgG subclasses and their implications for designing therapeutic monoclonal antibodies against infectious diseases.
Irani et al. (2015)
37 more

12 mentions without citation

Human IgG4: a structural perspective.
Davies et al. (2015)
11 more