4x8p

X-ray diffraction
2.2Å resolution

Crystal structure of Ash2L SPRY domain in complex with RbBP5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172252 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Set1/Ash2 histone methyltransferase complex subunit ASH2 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBL3 (Residues: 380-495, 539-598; Coverage: 28%)
Gene names: ASH2L, ASH2L1
Sequence domains: SPRY domain
Structure domains: Jelly Rolls
Retinoblastoma-binding protein 5 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15291 (Residues: 344-355; Coverage: 2%)
Gene names: RBBP5, RBQ3

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P212121
Unit cell:
a: 51.5Å b: 59.1Å c: 70.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.271
Expression systems:
  • Escherichia coli
  • Not provided