› 4x95

X-ray diffraction
3.08Å resolution

Crystal structure of fully glycosylated Lysosomal Phospholipase A2 in complex with methyl arachidonyl fluorophosphonate (MAFP)

Released:
DOI: 10.2210/pdb4x95/pdb
PDB entry 4x95 coloured by chain, front view.
PDB entry 4x95 coloured by chain, side view.
PDB entry 4x95 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of lysosomal phospholipase A2 and lecithin:cholesterol acyltransferase.
Glukhova A, Hinkovska-Galcheva V, Kelly R, Abe A, Shayman JA, Tesmer JJ
Nat Commun 6 6250 (2015)
PMID: 25727495

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal phospholipase A and acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 43.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NCC3 (Residues: 34-412; Coverage: 100%)
Gene names: LYPLA3, PLA2G15, UNQ341/PRO540
Sequence domains: Lecithin:cholesterol acyltransferase
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 6 x NAG
Ligand MAY 2 x MAY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 72.385Å b: 125.275Å c: 140.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.252
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Properties and Biotechnological Applications of Acyl-CoA:diacylglycerol Acyltransferase and Phospholipid:diacylglycerol Acyltransferase from Terrestrial Plants and Microalgae.
Xu et al. (2018)
3 more

2 mentions without citation

Structure and function of lysosomal phospholipase A2 and lecithin:cholesterol acyltransferase.
Glukhova et al. (2015)
1 more