› 4x9j

X-ray diffraction
1.41Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118259 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18146 (Residues: 335-423; Coverage: 16%)
Gene names: EGR1, KROX24, ZNF225
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*GP*(5CM)P*GP*TP*GP*GP*GP*(5CM)P*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*AP*(5CM)P*GP*CP*CP*CP*AP*(5CM)P*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.31 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
1 modified residue:
Ligand 5CM 4 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 44.009Å b: 55.989Å c: 128.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.2
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

DNA Methylation Readers and Cancer: Mechanistic and Therapeutic Applications.
Mahmood et al. (2019)
6 more

4 mentions without citation

Detecting and interpreting DNA methylation marks.
Ren et al. (2018)
3 more