› 4xaj

X-ray diffraction
3.55Å resolution

Crystal structure of human NR2E1/TLX

Released:
DOI: 10.2210/pdb4xaj/pdb
PDB entry 4xaj coloured by chain, front view.
PDB entry 4xaj coloured by chain, side view.
PDB entry 4xaj coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for corepressor assembly by the orphan nuclear receptor TLX.
Zhi X, Zhou XE, He Y, Searose-Xu K, Zhang CL, Tsai CC, Melcher K, Xu HE
Genes Dev 29 440-50 (2015)
PMID: 25691470

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126064 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; Nuclear receptor subfamily 2 group E member 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 576 amino acids
Theoretical weight: 63.54 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: Q9Y466 (Residues: 182-383; Coverage: 53%)
Gene names: JW3994, NR2E1, TLX, b4034, malE
Sequence domains:
Grunge, isoform J Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 18 amino acids
Theoretical weight: 2.09 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: M9PHT1 (Residues: 1814-1831; Coverage: 1%)
Gene names: Atro, CG6964, Dmel\CG6964, Dmel_CG6964, GUG, Gug, atro, gug, l(3)01323, l(3)03928, l(3)S146907, l(3)j5A3, l(3)rO116

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 72.26Å b: 130.743Å c: 308.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.272 0.314
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Visualizing the Architectures and Interactions of Nuclear Receptors.
Khorasanizadeh et al. (2016)
4 more

5 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
4 more