› 4xbr

X-ray diffraction
2.94Å resolution

In cellulo Crystal Structure of PAK4 in complex with Inka

Released:
DOI: 10.2210/pdb4xbr/pdb
PDB entry 4xbr coloured by chain, front view.
PDB entry 4xbr coloured by chain, side view.
PDB entry 4xbr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1.
Baskaran Y, Ang KC, Anekal PV, Chan WL, Grimes JM, Manser E, Robinson RC
Nat Commun 6 8681 (2015)
PMID: 26607847

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAK4-inhibitor INKA1; Serine/threonine-protein kinase PAK 4 Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 41.25 KDa
Source organism: Homo sapiens
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: Q96EL1 (Residues: 168-205; Coverage: 13%)
  • Canonical: O96013 (Residues: 278-591; Coverage: 53%)
Gene names: C3orf54, FAM212A, INKA1, KIAA1142, PAK4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 2 x MG
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P63
Unit cell:
a: 144.04Å b: 144.04Å c: 62.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.23
Expression system: Chlorocebus aethiops

Quick links

Citations

6 review citations

Where is crystallography going?
Grimes et al. (2018)
5 more

4 mentions without citation

An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1.
Baskaran et al. (2015)
3 more