› 4xh2

X-ray diffraction
2Å resolution

Crystal structure of human paxillin LD4 motif in complex with Fab fragment

Released:
DOI: 10.2210/pdb4xh2/pdb
PDB entry 4xh2 coloured by chain, front view.
PDB entry 4xh2 coloured by chain, side view.
PDB entry 4xh2 coloured by chain, top view.
Source organisms:
Primary publication:
Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA
J Mol Biol 427 2532-2547 (2015)
PMID: 26087144

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229964 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chains: A, C, E, G, H, J
Molecule details ›
Chains: A, C, E, G, H, J
Length: 228 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, D, F, I, K, L
Molecule details ›
Chains: B, D, F, I, K, L
Length: 217 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Paxillin Chains: a, c, e, g, h, j
Molecule details ›
Chains: a, c, e, g, h, j
Length: 18 amino acids
Theoretical weight: 1.94 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P49023 (Residues: 259-275; Coverage: 3%)
Gene name: PXN

Ligands and Environments

5 bound ligands:
Ligand GOL 20 x GOL
Ligand PO4 2 x PO4
Ligand ACT 1 x ACT
Ligand LDA 1 x LDA
Ligand ACE 2 x ACE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 117.58Å b: 138.12Å c: 223.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.
Derewenda et al. (2017)
2 more

1 mention without citation

Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Nocula-Lugowska et al. (2015)