› 4xhw

X-ray diffraction
2.85Å resolution

Crystal structure of Timeless_PAB domain in SeMet-labelled form

Released:
DOI: 10.2210/pdb4xhw/pdb
PDB entry 4xhw coloured by chain, front view.
PDB entry 4xhw coloured by chain, side view.
PDB entry 4xhw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Timeless Interacts with PARP-1 to Promote Homologous Recombination Repair.
Xie S, Mortusewicz O, Ma HT, Herr P, Poon RY, Poon RR, Helleday T, Qian C
Mol Cell 60 163-76 (2015)
PMID: 26344098

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-194017 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein timeless homolog Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 103 amino acids
Theoretical weight: 11.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNS1 (Residues: 1000-1098; Coverage: 8%)
Gene names: TIM, TIM1, TIMELESS, TIMELESS1
Sequence domains: Timeless PAB domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 66.597Å b: 100.083Å c: 149.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.253
Expression system: Escherichia coli

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Citations

19 review citations

ADP-ribosylation: new facets of an ancient modification.
Palazzo et al. (2017)
18 more