› 4xrs

X-ray diffraction
3.5Å resolution

Heterodimeric complex of transcription factors MEIS1 and DLX3 on specific DNA

Released:
DOI: 10.2210/pdb4xrs/pdb
PDB entry 4xrs coloured by chain, front view.
PDB entry 4xrs coloured by chain, side view.
PDB entry 4xrs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA-dependent formation of transcription factor pairs alters their binding specificity.
Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J
Nature 527 384-8 (2015)
PMID: 26550823

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218981 (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
Homeobox protein Meis1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 6.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00470 (Residues: 279-336; Coverage: 15%)
Gene name: MEIS1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
Homeobox protein DLX-3 Chains: G, I
Molecule details ›
Chains: G, I
Length: 56 amino acids
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60479 (Residues: 131-186; Coverage: 20%)
Gene name: DLX3
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(P*CP*AP*AP*TP*TP*AP*TP*CP*CP*TP*GP*TP*CP*AP*A)-3') Chain: M
Molecule details ›
Chain: M
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
DNA (5'-D(P*AP*CP*AP*AP*TP*TP*AP*TP*CP*CP*TP*GP*TP*CP*AP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.13 KDa
DNA (5'-D(P*GP*TP*TP*GP*AP*CP*AP*GP*GP*AP*TP*AP*AP*TP*TP*GP*TP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
DNA (5'-D(P*TP*TP*GP*AP*CP*AP*GP*GP*AP*TP*AP*AP*TP*TP*GP*T)-3') Chain: L
Molecule details ›
Chain: L
Length: 17 nucleotides
Theoretical weight: 5.28 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 69.636Å b: 69.845Å c: 116.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.343 0.33 0.359
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

54 review citations

Ever-Changing Landscapes: Transcriptional Enhancers in Development and Evolution.
Long et al. (2016)
53 more

1 mention without citation

DNA-guided transcription factor cooperativity shapes face and limb mesenchyme.
Kim et al. (2023)